In bacteria and viruses general recombination is carried out by the products of rec genes such as RecA protein. Many other proteins apart from the recombinase are involved in regulating the reaction. outcome. place. Site-specific recombination (SSR) involves specific sites for the catalyzing action of special enzymes called recombinases. [26][27][28] Genetic location of the floxed sequence affects recombination efficiency as well probably by influencing the availability of DNA by Cre recombinase. Cre/ lox is usually used to make knockout alleles, but it can also be used to activate gene expression. It is implemented both in eukaryotic and prokaryotic systems. Models thus obtained are more likely to mimic the physiological situation. The Cre activity can be induced by applying inducer to initiate the recombination which will lead to self-excision of cre and the marker gene (see below) Marker gene Trait gene loxP loxP Chemical-induced cre gene chemical Trait gene loxP Genomic targeting with a positive-selection lox . Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. With genomic tumor testing, a negative result is not always definitive. The Cre enzyme leads to the recombination between lox sites removing the marker gene along with one lox site. This site needs JavaScript to work properly. As with any other technology or research tool, it has limitations and pitfalls that need to be considered while planning experiments or interpreting results. To account for this, alternate mutant versions of the loxP site have been created, which contain a unique asymmetric spacer "NNNTANNN", where "N" indicates which bases may vary from the canonical sequence. bioRxiv. It is implemented both in eukaryotic and prokaryotic systems. lox. Does Addgene accept orders by fax, phone or email? In more recent years, however, this view has been challenged, and most of the current models for Int, Xer, and Flp recombination involve only limited branch migration (13 base pairs of the Holliday intermediate), coupled to an isomerisation event that is responsible for switching the strand cleavage specificity. The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. mutant/transgenic mice. First, let us focus on the Cre-loxP system which requires a Cre The Cre-Lox recombination system is derived from P1 Bacteriophages and uses a specific recombinase protein (Cre) that targets a unique DNA sequence (loxP) for splicing. (In a future post, I will tackle the breeding schemes for using the system to turn transgenes on or off. > g ` a b c d e f n T%O)JPNG
This reaction is aided with the endonuclease activity of an enzyme. Now customize the name of a clipboard to store your clips. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is one of the most powerful tools in the mouse geneticists toolbox. This plasmid is an autonomously replicating, circular plasmid of 6,318 base pairs, which exists in many copies in most strains of S. cerevisiae (q.v.). This has been largely due to . Bacteriophage P1 site-specific recombination. For many years, it was thought that when the Holliday junction intermediate was formed, the branch point of the junction (where the strands cross over) would be located at the first cleavage site. PubMed Central PMCID:PMC281709. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through CreloxPmediated gene targeting. This reaction links the recombinase protein to the DNA via a phospho-tyrosine linkage. Scientists have developed ways to tightly control Cre expression and to optimize Cre expression once it is induced. GGS Medical College/Baba Farid Univ.of Health Sciences. The SlideShare family just got bigger. cre-lox and cre recombinases in Mouse Genome Informatics (MGI): Module 2 MouseGenomeInformatics 17.7k views Knock out technology (final) Dr Vijayata choudhary 22.2k views Gene knockout GGS Medical College/Baba Farid Univ.of Health Sciences. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur. The Cre-lox system serves several functions in the phage: it circularizes the phage DNA into a plasmid, separates interlinked plasmid rings so they are passed to both daughter bacteria equally and may help maintain copy numbers through an alternative means of replication. This dimer then binds to a dimer on another lox site to form a tetramer. 5 Phosphate ends are usually the substrates for this reaction, thus extended 3 regions remain. Cre-lox, To generate mice that are heterozygous for aloxP-flanked allele and hemizygous/heterozygous for thecretransgene, mate a homozygousloxP-flanked mouse of interest to a cre transgenic mouse strain (see Figure 1 below). PubMed, CRISPR Expression Systems and Delivery Methods. The system consists of two components derived from the P1 bacteriophage: the Cre recombinase and a loxP recognition site. A number of conserved site-specific recombination systems have been described in both prokaryotic and eukaryotic organisms. We've updated our privacy policy. 10. This provides the advantage that the Cre recombinase is active for a short time. ERT2-Cre-ERT2 - Tamoxifen inducible. Site-specific recombination is, thus, the enzyme-mediated cleavage and ligation of two defined deoxynucleotide sequences. CreER(T2) resides within the cytoplasm and can only translocate to the nucleus following tamoxifen administration, allowing tight temporal control of recombination. 1 See, for example, Lyznik et al., Site-Specific Recombination for Genetic Engineering in Plants, Plant Cell Rep, 21:925-932 (2003) and WO 99/25821, which are hereby incorporated by reference. Cre-RMCE is functional in a two-step mechanism and with good efficiency in Ae. 3. Cre recombinase can recognize and bind to the loxP site, The exact sequence is given below; 'N' indicates bases which may vary, and lowercase letters indicate bases that have been mutated from the wild-type. In the absence of tamoxifen, CreER will result in the shuttling of the mutated recombinase into the cytoplasm. Since both the transposon and the Cre- lox recombineering systems were applied to diverse hosts, CRAGE enabled insertion of large biosynthetic gene clusters into 25 diverse gammaproteobacterial species. In this context we created four target site lines for RMCE and evaluated their fitness costs. If the two loxP sites are in the same orientation, the floxed sequence (sequence flanked by two loxP sites) is excised; however if the two loxP sites are in the opposite orientation, the floxed sequence is inverted. It is a member of a large family of tyrosine recombinases that includes several other familiar recombinases, such as Flp and Int. Such conditional lineage tracing has proved to be extremely useful to efficiently and specifically identify vascular smooth muscle cells (VSMCs) and VSMC-derived cells and has been used to test effects on VSMC and VSMC-derived cells in vivo.[32][33][34][35][36][37]. Missirlis PI, Smailus DE, Holt RA. (RBE); the two RBE are 13bp You may not be able to create an account or request plasmids through this website until you upgrade your browser. This event can have three general outcomes based on the location and orientation of the loxP sites: Inversion: If the loxP sites are on the same DNA strand and are in opposite orientations, recombination results in an inversion and the region of DNAbetween the loxP sites is reversed. I. Recombination between loxP sites. Linker, Adaptor, Homopolymeric Tailing & Terminal Transferase, Transcriptional and post transcriptional regulation of gene expression, Molecular biology of the gene ch 13 rna splicing part1, Alternative splicing : mechanism and regulation, POST TRANSCRIPTIONAL MODIFICATIONS IN EUKARYOTES, Nepalgunj Medical College and Teaching Hospital, govt .science college ,tumkur,( govt.estab), artificial or synthetic transcription factor for regulation of gene expression, Enzyme Kinetics and thermodynamic analysis, Chromatin, Organization macromolecule complex, eukaryotic translation machinery by kk sahu, Easy-handling carbon nanotubes decorated poly(arylene ether nitrile).pdf. Activate your 30 day free trialto continue reading. 1992. This 3 OH group is highly unstable, and the strand on which it is present must find its complement. Usually loxP sites come in pairs for genetic manipulation. Sauer, B. and Henderson, N. 1988. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR, AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR, AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR, AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA, pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert, pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2, pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre, hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean, pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP, Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientists View, CREM and CMV-STOP-luc cassette, separated by insulator, Cre-EGFP fusion in a promoterless vector with several available restriction sites, tet inducible Cre-GFP fusion, metallothionein MT-I region including the polyadenylation site and several introns, Cre-ERT2 with loxp cassette; Targeting vector for Rosa26 locus, CREM fused to mutant AR ligand binding domain, Cre fused to HIV-TAT - promotes cellular uptake of recombinant Cre, Cre-Myc, Expressed in rod photorecetor cells in the retina. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1. Please note: Your browser does not support the features used on Addgene's website. Multiple variants of loxP,[41] in particular lox2272 and loxN, have been used by researchers with the combination of different Cre actions (transient or constitutive) to create a "Brainbow" system that allows multi-colouring of mice's brain with four fluorescent proteins. Bethesda, MD 20894, Web Policies An attP site on the phage exchanges segments with an attB site on the bacterial DNA. [2], Cre-Lox recombination is a special type of site-specific recombination developed by Dr. Brian Sauer and patented by DuPont that operated in both mitotic and non-mitotic cells, and was initially used in activating gene expression in mammalian cell lines. Click here to review the details. A key advantage of the system is that Cre acts independently of any other accessory proteins or co-factors, thus allowing for broad applications in a variety of experiments. Explaining these schemes can be difficult, and I swear that I can sometimes feel customers eyes cross while Im trying to explain them over the telephone. Now researchers are using sequencing technology to better, Assessing patient health risks with limited or unknown family history, Using the power of sequencing on our smallest organisms. 2 D ). Mol Urol. Sternberg, N. and Hamilton, D. 1981. It appears that you have an ad-blocker running. The versatile properties of Cre recombinase make it ideal for use in many genetic engineering strategies. Protein-protein interactions drive and direct strand exchange. The use of the Cre/loxP system for generating tissue specific or inducible knockouts is a powerful tool for mouse genetics, but it is only one piece of the total picture. How can I be notified when a plasmid from a specific lab or paper is available? GENE KNOCKOUT USING THE CRE-LOXP SYSTEM The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. Nov 2, 2015. Is LysM-Cre a good candidate Cre for knocking out Atg5 gene in mice. Cre-lox Systems Cre-lox (floxed) systems for gene deletion ( click here for non-floxed and earlier floxed tagging vectors) General Comments The baseline reference for this system is CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei (2008) Scahill MD, Pastar I & Cross GAM. Again, only one generation of breeding is required. Fill out our Suggest a Plasmid form or e-mail [emailprotected] to help us improve this resource! DNA Replication in eukaryotes and prokaryotes. Commonly used gene replacement strategies introduce selectable markers into the genome to facilitate selection of genetic mutations that may cause growth retardation. The products of the recombination event depend on the relative orientation of these asymmetric sequences. Faust TE, Feinberg PA, O Connor C, Kawaguchi R, Chan A, Strasburger H, Masuda T, Amann L, Knobeloch KP, Prinz M, Schaefer A, Schafer DP. A comparative analysis of microglial inducible Cre lines. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. eCollection 2022. You can search the table for the promoter, fusion, or expression system of choice. Cre reporter strains can also be used to generate a source of labeled tissues/organs, which allow researchers to track cells in transplant or adoptive transfer experiments. By undergoing Cre recombination, the region floxed by the loxP sites will become inverted, [12] this process is not permanent and can be reversed. Cre recombinase proteins bind to the first and last 13 bp regions of a lox site forming a dimer. Department of Biotechnology It has been even more useful in the Neuroscience field, to understand and study effects of genes and proteins in the brain on different tissues/areas at a time. 18 This review provides an overview of the cre-loxP system and its various permutations. NNNTANNN", where "N" indicates which bases may vary from the canonical sequence, Sternberg, N. and Hamilton, D. 1981. By accepting, you agree to the updated privacy policy. 2007;(178):29-48. doi: 10.1007/978-3-540-35109-2_2. [7] Combining these advances in collaboration, the laboratories of Drs. Cre- loxP cre loxP GFP cre GFP cre cre cre Energy is not compromised, since the protein-DNA linkage makes up for the loss of the phosphodiester bond, which occurred during cleavage. PubMedPMID:6276558. For example, mating the transgenic strain in Figure 1 to a strain that expresses Cre recombinase in mammary tissue produces double transgenic offspring that express theKras oncogene only in the mammary glands (Figure 5). The P1 plasmid is relatively large (90Kbp) and hence exists in a low copy number - usually one per cell. NIH Blueprint for Neuroscience Research has created several hundreds of Cre driver mouse lines which are currently used by the worldwide neuroscience community. The cre-loxP-mediated recombination system (the "cre-loxP system") is an integral experimental tool for mammalian genetics and cell biology. It was unclear whether only one allele in each T-cell or 50% of T cells had 100% deletion in both alleles. Inducible Cre activation is achieved using CreER (estrogen receptor) variant, which is only activated after delivery of tamoxifen. A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination. The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system. Cells. The asymmetry in the core sequence gives the loxP site directionality, and the canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. This recombinase comes from the P1 bacteriophage. sharing sensitive information, make sure youre on a federal Matsumura T, Watanabe T, Totsugawa T, et al. Upon Cre driven recombination, the stop cassette is excised, allowing reporter genes to express specifically in cells in which the Cre expression is being driven by the cell-specific marker promoter. Chai W, Hao W, Liu J, Han Z, Chang S, Cheng L, Sun M, Yan G, Liu Z, Liu Y, Zhang G, Xing L, Chen H, Liu P. JBMR Plus. If you run into any problems registering, depositing, or ordering please contact us at [emailprotected]
Engineered lox variants which differ on the spacer region tend to have varied but generally lower recombination efficiency compared to wildtype loxP, presumably through affecting the formation and resolution of recombination intermediate.[25]. Subsequent cleavage and rejoining cause DNA strands to exchange their segments. Cell Culture Media, Introduction to the Cre-Lox Recombination System, AAALAC-accredited and OLAW assured animal centers, Specific-pathogen-free (SPF) animal health status exceeds requirements of most facilities, Top-tier customer support: free consultations and quotes. At a DNA level many types of manipulations can be carried out. 2. Tap here to review the details. must be introduced artificially. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. If you are looking for the right Cre-expressing strain for your experiments, you can search for strains at the JAX Cre Repository or through the Cre Portal at MGI. There is a problem with the plasmid I received. and transmitted securely. recombination; recombination between two loxP sites can induce a deletion. Common construct types include: Note: Through a partnership with genOway, Addgene is able to distribute materials containing FLEx technology. Conditional gene inactivation can be achieved with the Cre/ lox system in which the bacteriophage Cre recombinase promotes site-specific recombination at compatible loxP sites engineered in a gene of interest ( Sauer and Henderson, 1988 ). These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally. Considering users' convenience to optimize the amount of blue light needed for single genomic Cre-lox recombination, we infected PA-Cre 3.0 to MEFs isolated from Ai14: Floxed-tdTomato . The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location of the loxP sites determines how the genetic material will be rearranged. Migration of the branch point to the second cleavage site would then somehow trigger the second half of the pathway. Both the Cre recombinase, and the loxP sites are not present in the natural mouse, and as such [28] Failure to activate both recombination events simultaneously confounds the interpretation of cell fate mapping results. Here, we present a simple method that combines the use of CRISPR-Cas9 and Cre-LoxP for the Recombinant modified Cre recombinase protein translocates into the cytoplasm and nuc The FLP-FRT technology can be an effective alternative to Cre-lox, and has also been used in conjunction with it, allowing for two separate recombination events to be controlled in parallel. National Library of Medicine Free access to premium services like Tuneln, Mubi and more. Chief among these is Cre recombinase, discovered in the P1 bacteriophage and named as such because it c auses re combination (Cre) between specific DNA sequences referred to as loxP (locus of crossover (x) in P1) sites, each of which are 34 base pairs in length 1. Site-specific recombination is also an important process that viruses, such as bacteriophages, adopt to integrate their genetic material into the infected host. If loxP sites are on different chromosomes it is possible for translocation events to be catalysed by Cre induced recombination. In Cre-DIO system, there are two pairs of lox sites (lox site 1 and lox site 2) flanking inverted GOI and reporter gene. The Cre-lox system is used to introduce deletions, insertions, translocations, and inversions at a specific genomic locus. The technology called Cre-lox recombination relies on the use of an enzyme called Cre recombinase which can bind, cut and recombine DNA at specific sites that are inserted in pairs in the DNA. Do you have any tips or tricks, or clever cre-dependent techniques? Many Cre constructs also contain fluorescent labels that indicate if recombination has occurred, allowing for direct comparison of Cre+ and Cre- cells. The Cre-lox recombination system prevents these situations by unlinking the rings of DNA by carrying out two recombination events (linked rings -> single fused ring -> two unlinked rings). We've encountered a problem, please try again. The Cre- lox recombination system is derived from the bacteriophage P1. You can read the details below. Read Addgene's Blog on Cre alternatives from Gerald Rubin's lab. At both of the DNA duplexes, the bonding of the phosphate group to tyrosine residues leave a 3 OH group free in the DNA backbone. . To rule out the possibility that the gene recombination efficacy was insufficient for CK19;PTEN / mice to develop tumors, we administered the same dose of TAM for 3 consecutive days by oral gavage, but no ampullary tumors were observed after 90 days (n = 4). What do I need to know about the customs and importation process for my country? Visualizing Cathepsin K-Cre Expression at the Single-Cell Level with GFP Reporters. Clipping is a handy way to collect important slides you want to go back to later. doi: 10.1002/jbm4.10706. 2 D ). Addgene is a nonprofit plasmid repository. 2001 Summer;5(2):81-4. doi: 10.1089/109153601300177600. Conversely, pSicoR-p53 generated a p53 knock-down that was reversed upon Ad-Cre infection ( Fig. By expressing Cre at specific times or locations, you can precisely control expression of your gene of interest. The Cre-lox system, derived from P1 bacteriophage, is a potent and specific system for controlling gene expression. Plasmid Elements, Cre/lox is usually used to make knockout alleles, but it can also be used to activate gene expression. Depending on the By The invention relates to the seeds of soybean cultivar 03070412, to the plants of soybean cultivar 03070412, to the plant parts of soybean cultivar 03070412, and to methods for producing progeny of soybean cultivar 03070412. See, Cre activates gene of interest, removes Neo and stop cassette; Contains flanking arms for Rosa26 integration; See similar plasmid. It is also proposed that rolling circle replication followed by recombination will allow the plasmid to increase its copy number when certain regulators (repA) are limiting. 1. Maize hybrid X13C737 is an invention by Andrew Jon Ross, Lisbon IA UNITED STATES. Cre/loxP-mediated chromosome engineering of the mouse genome. Because the controls will not have Cre recombinase, any phenotypic differences between them and the Cre/lox mice should be due to the deleted gene in the Cre/lox mice. Here is Part 2!). However, this approach does not provide capabilities beyond insertion, such as gene deletions or modulated gene expression. In particular, the clear demonstration of its usefulness in precisely defining the complex relationship between specific cells/circuits and behaviors for brain research,[12] has promoted the NIH to initiate the NIH Blueprint for Neuroscience Research Cre-driver mouse projects in early 2000. Clipping is a handy way to collect important slides you want to go back to later. Disclaimer. See more Base pair. Let us know in the comments! Siegel04.. Bacteriophage P1 uses a site-specific recombination system that is responsible for partitioning newly synthesized genomic copies during replication Abremski, Hoess.This system is composed of a 38-kD phage-encoded Cre recombinase that mediates symmetrical recombination between two 34-bp loxP sites Abremski, which are recreated after . In previous posts for our Plasmids 101 series, we examined a number of important plasmid elements promoters, origins of replication, protein tags, and antibiotic resistance markers(just to name a few). By accepting, you agree to the updated privacy policy. Expressed in chondrocytes, mCherry and Cre-ERT2 - Tamoxifen inducible; Targeting vector for Pax7, Venus and Cre-ERT2 with MCS for inserting promoter, Emerald and Cre-ERT2 with MCS for inserting promoter, TFP and Cre-ERT2 with MCS for inserting promoter, tdTomato and Cre-ERT2 with MCS for inserting promoter, Venus, Cre-ERT2, targeting vector with MCS for homology arms, Emerald, Cre-ERT2, targeting vector with MCS for homology arms, TFP, Cre-ERT2, targeting vector with MCS for homology arms, tdTomato, Cre-ERT2, targeting vector with MCS for homology arms, GAG-Cre fusion; for the production of VLPs loaded with CRE protein, sfGFP-iCre-ERT2 (PAPGSTM N-terminus, unskippable linker) - Tamoxifen inducible, sfGFP-iCre-ERT2 (PAPGSTM N-terminus, GSAx9 linker) - Tamoxifen inducible, iCre-ERT2 (PAPGSTMA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PV N-terminus) - Tamoxifen inducible, iCre-ERT2 (PVV N-terminus) - Tamoxifen inducible, iCre-ERT2 (PVGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PA N-terminus) - Tamoxifen inducible, CreLite - Red light-inducible Cre; N terminus Cre fused to PIF6, CreLite - Red light-inducible Cre; C terminus Cre fused to PhyBdelta, CreLite system components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit), CreLite; Tol2 destination vector with mTagBFP2, CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer vector, CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector, Cre recombinase split with Vivid photodimers, Cre-ERT2 - Tamoxifen inducible; AAV donor vector, iCre-ERT2 (PAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAGSAS N-terminus) - Tamoxifen inducible, N-terminal Cre component split with Vivid photodimers, C-terminal Cre component split with Vivid photodimers, Split Cre fused to nuclear localized wild-type VVD, Retinal ganglion cell-specific expression of Cre, Cre activates shRNA expression, removal of EGFP; See also similar plasmids, Cre turns off shRNA expression, removal of EGFP; See also similar plasmids, Cre activates gene of interest by removing Stop sequences; Puro selection. Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. Cre-Lox recombination is a sophisticated site-specific recombinase technology that allows DNA modification to be targeted to a specific cell type or to be triggered by a specific external stimulus. "Site-specific recombination of DNA in eukaryotic cells", "Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae", "Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1", "Tissue- and site-specific DNA recombination in transgenic mice", "T-cell-specific deletion of a polypeptide N-acetylgalactosaminyl-transferase gene by site-directed recombination", "Cre-Lox Neurogenetics: 20 Years of Versatile Applications in Brain Research and Counting", "Subregion- and cell type-restricted gene knockout in mouse brain", "The essential role of hippocampal CA1 NMDA receptor-dependent synaptic plasticity in spatial memory", http://www.neuroscienceblueprint.nih.gov/factSheet/CreDriver.htm, "Skeletal and CNS defects in Presenilin-1-deficient mice", "Deficient neurogenesis in forebrain-specific presenilin-1 knockout mice is associated with reduced clearance of hippocampal memory traces", "Yeast two-hybrid interaction partner screening through in vivo Cre-mediated Binary Interaction Tag generation", "Cyclization recombinase [Escherichia coli] - Protein - NCBI", "Targeted integration of DNA using mutant lox sites in embryonic stem cells", "A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination", "A genetic strategy for stochastic gene activation with regulated sparseness (STARS)", "Engineering mouse chromosomes with Cre-loxP: range, efficiency, and somatic applications", "Non-parallel recombination limits Cre-LoxP-based reporters as precise indicators of conditional genetic manipulation", "Genetically engineered mouse models in cancer research", "Spontaneous recombinase activity of Cre-ERT2 in vivo", "Epigenetic Regulation of Vascular Smooth Muscle Cells by Histone H3 Lysine 9 Dimethylation Attenuates Target Gene-Induction by Inflammatory Signaling", "Extensive Proliferation of a Subset of Differentiated, yet Plastic, Medial Vascular Smooth Muscle Cells Contributes to Neointimal Formation in Mouse Injury and Atherosclerosis Models", "Previously differentiated medial vascular smooth muscle cells contribute to neointima formation following vascular injury", "KLF4-dependent phenotypic modulation of smooth muscle cells has a key role in atherosclerotic plaque pathogenesis", "Lineage tracing of cells involved in atherosclerosis", "Disease-relevant transcriptional signatures identified in individual smooth muscle cells from healthy mouse vessels", Introduction to Cre-lox technology by the "Jackson Laboratory", https://en.wikipedia.org/w/index.php?title=Cre-Lox_recombination&oldid=1135942402, This page was last edited on 27 January 2023, at 20:38.
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